Analysis Note
Positive ControlLiver sections, HT1080 cells, HepG2 cells
Application
ELISA (0.5-1 g/ml)Flow Cytometry (see application references)Frozen Sections (1-5 g/ml)Immunoblotting (1-5 g/ml, see application references)Immunocytochemistry (1-5 g/ml, IF)Immunoprecipitation (1-2 g/sample)
General description
Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
Immunogen
Epitope: first cysteine-rich ligand binding domain
a trypic digest of bovine low density lipoprotein receptor
Bovine
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127. van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443. Russell, D.W., et al. 1984. Cell37, 577.Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635. Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261. Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664. Goldstein, J.L., et al. 1979. Nature279, 679.
Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
Packaging
100 µg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: