Analysis Note

Positive ControlLiver sections, HT1080 cells, HepG2 cells

Application

ELISA (0.5-1 g/ml)Flow Cytometry (see application references)Frozen Sections (1-5 g/ml)Immunoblotting (1-5 g/ml, see application references)Immunocytochemistry (1-5 g/ml, IF)Immunoprecipitation (1-2 g/sample)

General description

Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.

This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.

Immunogen

Epitope: first cysteine-rich ligand binding domain

a trypic digest of bovine low density lipoprotein receptor

Bovine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127. van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443. Russell, D.W., et al. 1984. Cell37, 577.Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635. Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261. Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664. Goldstein, J.L., et al. 1979. Nature279, 679.

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺² with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)